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plasmid containing the neomycin resistance gene pci-neo  (Promega)

 
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    Structured Review

    Promega plasmid containing the neomycin resistance gene pci-neo
    Plasmid Containing The Neomycin Resistance Gene Pci Neo, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+containing+the+neomycin+resistance+gene+pci-neo/plasmid+containing+the+neomycin+resistance+gene+pci+neo/pmc04421896-93-6-15
    Average 90 stars, based on 1 article reviews
    plasmid containing the neomycin resistance gene pci-neo - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Transforming Growth Factor-β Induction of Smooth Muscle Cell Phenotpye Requires Transcriptional and Post-transcriptional Control of Serum Response Factor
    Article Snippet: Creation and Characterization of 10T1/2 Stable Transfectants— 10T1/2 cells were transfected, via electroporation, with 5 g of linearized HA-tagged expression plasmid containing no cDNA (vector control), 5 g of linearized wild type (wt) SRF cDNA, or 5 g of linearized SRF cDNA that contains a 3-bp substitution in the region known to mediate DNA binding, yielding a dominant negative SRF protein (dnSRF; described previously in Ref. 36). .. All cells were cotransfected with a plasmid containing the neomycin resistance gene (pCI-neo; Promega) at 1⁄10 the concentration of experimental vector. .. Twelve stable transfectant clones were generated from each experimental group (vector, wtSRF, dnSRF) via selection in normal growth media containing 1000 g/ml G418; thereafter, stable clones were maintained in 500 g/ml G418.

    Article Title: Transforming Growth Factor- β Induction of Smooth Muscle Cell Phenotpye Requires Transcriptional and Post-transcriptional Control of Serum Response Factor
    Article Snippet: Creation and Characterization of 10T1/2 Stable Transfectants 10T1/2 cells were transfected, via electroporation, with 5 μ g of linearized HA-tagged expression plasmid containing no cDNA (vector control), 5 μ g of linearized wild type (wt) SRF cDNA, or 5 μ g of linearized SRF cDNA that contains a 3-bp substitution in the region known to mediate DNA binding, yielding a dominant negative SRF protein (dnSRF; described previously in Ref. 36 ). .. All cells were cotransfected with a plasmid containing the neomycin resistance gene (pCI- neo ; Promega) at 1/10 the concentration of experimental vector. .. Twelve stable transfectant clones were generated from each experimental group (vector, wtSRF, dnSRF) via selection in normal growth media containing 1000 μ g/ml G418; thereafter, stable clones were maintained in 500 μ g/ml G418.

    Concentration Assay:

    Article Title: Transforming Growth Factor-β Induction of Smooth Muscle Cell Phenotpye Requires Transcriptional and Post-transcriptional Control of Serum Response Factor
    Article Snippet: Creation and Characterization of 10T1/2 Stable Transfectants— 10T1/2 cells were transfected, via electroporation, with 5 g of linearized HA-tagged expression plasmid containing no cDNA (vector control), 5 g of linearized wild type (wt) SRF cDNA, or 5 g of linearized SRF cDNA that contains a 3-bp substitution in the region known to mediate DNA binding, yielding a dominant negative SRF protein (dnSRF; described previously in Ref. 36). .. All cells were cotransfected with a plasmid containing the neomycin resistance gene (pCI-neo; Promega) at 1⁄10 the concentration of experimental vector. .. Twelve stable transfectant clones were generated from each experimental group (vector, wtSRF, dnSRF) via selection in normal growth media containing 1000 g/ml G418; thereafter, stable clones were maintained in 500 g/ml G418.

    Article Title: Transforming Growth Factor- β Induction of Smooth Muscle Cell Phenotpye Requires Transcriptional and Post-transcriptional Control of Serum Response Factor
    Article Snippet: Creation and Characterization of 10T1/2 Stable Transfectants 10T1/2 cells were transfected, via electroporation, with 5 μ g of linearized HA-tagged expression plasmid containing no cDNA (vector control), 5 μ g of linearized wild type (wt) SRF cDNA, or 5 μ g of linearized SRF cDNA that contains a 3-bp substitution in the region known to mediate DNA binding, yielding a dominant negative SRF protein (dnSRF; described previously in Ref. 36 ). .. All cells were cotransfected with a plasmid containing the neomycin resistance gene (pCI- neo ; Promega) at 1/10 the concentration of experimental vector. .. Twelve stable transfectant clones were generated from each experimental group (vector, wtSRF, dnSRF) via selection in normal growth media containing 1000 μ g/ml G418; thereafter, stable clones were maintained in 500 μ g/ml G418.



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    Promega plasmid containing the neomycin resistance gene pci-neo
    Plasmid Containing The Neomycin Resistance Gene Pci Neo, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+containing+the+neomycin+resistance+gene+pci-neo/plasmid+containing+the+neomycin+resistance+gene+pci+neo/pmc04421896-93-6-15
    Average 90 stars, based on 1 article reviews
    plasmid containing the neomycin resistance gene pci-neo - by Bioz Stars, 2026-10
    90/100 stars
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